Applications : Fluorescence Spectrophotometer(FL)

NO. Title FL33 ETHANOL MEASUREMENT AND ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY The F-7000 fluorescence spectrophotometer achieves a high sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min) wavelength scan at the highest level in its class, with a wide dynamic range up to 6 digits. Furthermore, it is applied to research, development, and quality control in a broad range of fields, such as industrial materials, life sciences, and biotechnology. The 4-turret accessory used in this experiment automatically measures, by computer control, the time course of the fluorescence intensity at regular time intervals through 4 rotating cells installed in a turret. A reaction trace for an enzyme is used to measure the time course of fluorescence intensity in plural samples. Previously, the time course was measured for one sample at a time, so the measurement time required was proportional to the number of samples. Application of the automatic 4-turret accessory reduces measurement time. An enzyme assay was applied to ethanol measurement in this experiment. The amount of ethanol in the fish is increased according to a decline of the freshness, so this measurement is referred to as the freshness index. Ethanol is usually measured with a spectrophotometer. When ethanol is present in trace amounts, a low concentration measurement is performed with a fluorescence spectrophotometer. In this experiment, the general enzyme assay reagent “F-kit ethanol (J.K.International)” was used. In addition to the enzyme assay, a spectrophotometer was used to measure ethanol amounts. The analysis conditions were optimized, and an analytical curve for a standard solution was created.  Model: F-7000 FL32 FLUORESCENCE SPECTRUM MEASUREMENT METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT - SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT MATERIAL - Model:  F-7000 Keywords: Fluorescence spectrum, Spectrum calibration, Instrumental response, Rhodamine B, Quinine sulfate, Standard material, GLP/GMP program FL31 DETECTION OF INTERACTION BETWEEN RECEPTOR PROTEINS DEPENDING ON BIOLUMINESCENCE RESONANCE ENERGY TRANSFER Model: F-4500 Keywords: Fluorescence, Luminescence, HEK Cell, Protein Interaction, Receptor, Bioluminescence Resonance Energy Transfer, BRET, FRET, Luminescence resonance energy transition, GFP, YFP, ATP, G-protein FL30 FLUORESCENCE POLARIZATION METHOD APPLIED TO BIOCHEMICAL STUDY - DETERMINATION OF PCR PRODUCT - Model: F-4500 Keywords: Fluorescence polarization accessory, Biochemistry, DNA, RNA, PCR FL29 FLUORESCENCE POLARIZATION METHOD APPLIED TO BIOCHEMICAL STUDY Model: F-4500 Keywords: Fluorescence polarization method, Biochemistry, Molecular weight, Enzyme activity, Trypsin, Cytoplasmic steaming FL28 AUTOMATIC FLUORESCENCE SPECTROPHOTOMETRIC ASSAYS FROM MICROTITER PLATES Model: F-4500 Keywords: Trace sample, Mictotiter plates, Enzyme activity measurement, Automation FL27 HIGHLY SENSITIVE DETERMINATION OF TERBIUM IN YTTRIUM OXIDE Model: F-4500 Keywords: Fluorescense determination, Terbium, Three-wavelength calculation FL26 MEASUREMENT EXAMPLES OF FLUORESCENT WHITE SAMPLES Model: F-4500 Keywords: Fluorescent whitening agent, Whiteness, Spectral reflected radiance factor, Spectral total radiance factor FL25 AN ADVANCED FLUORESCENCE SPECTROPHOTOMETRIC MEASURING SYSTEM FOR RAPID ACQUISITION OF THREE DIMENSIONAL SPECTRA Model: F-4500 Keywords: 3-dimensional fluorescence spectra, High speed data acquisition, Petroleum, Serotonin FL24 THE DETERMINATION OF CORRECTED EXCITATION SPECTRA, CORRECTED EMISSION SPECTRA AND QUANTUM YIELDS Model: F-4010 Keywords: Spectrum correction, Correction factor, Quantum yield, Quinine sulfate, Emission spectra, Excitation spectra FL23 MEASUREMENT OF COUMARIN BY FLUORESCENCE ANALYSIS Model: F-2000 Keywords: Coumarin, Kerosene, Light oil FL22 MEASUREMENT OF CYTOSOLIC Ca2+ IN THE INITIAL GROWTH OF MOUSE FIBROBLAST CELLS Model: F-2000 Keywords: PIP2, PDE, IP3, BALB/C 3T3

	F-7000 Fluorescence Spectrophotometer