Applications : Liquid Chromatograph Mass Spectrometer (LCMS)

We introduce you to plenty of data on analytical methods and their results using our Liquid Chromatograph Mass Spectrophotometer.

	Technical NOTE	Applications NOTE
	Technical NOTE NO. Title NF/TN(E)-003  High throughput ECD (QuECD) analysis in LIT-q-TOF Mass Spectrometer (PDF, 877KB)  A new linear ion trap-q-TOF mass spectrometer equipped with QuECD, the NanoFrontier eLD, enables LC-ECD-MS/MS analysis. The results shown here demonstrate the NanoFrontier eLD's capability to analyze post-translational modifications and to perform de novo sequencing of large peptides.  Model:NanoFrontier eLD NF/TN(E)-002  Electron Capture Dissociation in a Radio-Frequency Linear Ion Trap (PDF, 752KB)  Here we describe a new compact device for electron capture dissociation (ECD) analysis of large peptides and post-translational modifications of proteins, which can be difficult to analyze via conventional dissociation techniques such as collision induced dissociation (CID).  Model:NanoFrontier NF/TN(E)-001  A New Tool for Mass Analysis of Unknown Molecules: High-Resolution Multi-Step Tandem Mass Spectrometer with Wide Dynamic Range Quantitative Analysis (PDF, 344KB)  Mass spectrometers are effective tools to identify and quantify unknown molecules, such as disease-related proteins and small molecules in pharmaceutical research and medical diagnosis. In addition, mass spectrometry can be particularly powerful when analyzing molecules with complex structures, such as post-translationally-modified proteins.  Model:NanoFrontier

Applications NOTE NO. Title NF/AN(E)-013  Phosphoproteome analysis using Electron Capture Dissociation tandem mass spectrometry (PDF, 449KB)  ECD induces cleavage of peptide backbone bonds of multiplycharged peptides and proteins via low energy (<1 eV) electron capture. ECD proceeds via a chargereduced radical intermediate, which fragments at peptide backbone N-C bonds yielding extensive c and z ions without the loss of labile modifications. ECD therefore allows both peptide identification and precise determination of the PTM location.  Model:NanoFrontier NF/AN(E)-012  Characterization of a Complex Protein Mixture using nanoLC-LIT-TOF Mass Spectrometry : Advances in Two Technologies Come Together to Create a Highly Effective Protein Profiling Instrument (PDF, 637KB)  Hybrid MS analyzers increase the speed and accuracy of mass analysis, allowing for a more comprehensive interrogation of peptides as they are eluted from the column. Here, the authors utilize an instrument that combines the advantages of both nanoscale liquid chromatography and a linear ion trap time-of-flight (LIT-TOF) hybrid mass analyzer to characterize Universal Proteomics Standard 1 (Sigma-Aldrich), an established standard complex mixture of proteins.  Model:NanoFrontier NF/AN(E)-011  New proteome platform of 2DICAL and its clinical application (PDF, 647KB)  2DICAL converts the LCMS data to a 2-dimensional image where the axes of mass to charge ratio (m/z) and retention time (RT) correct the time fluctuation of RT according to the correlation coefficient of each spectrum. This technique makes it possible to compare different samples without isotope labeling because each coordinate representing m/z and RT with a variable intensity indicates a peak originating from a different peptide.  Model:NanoFrontier NF/AN(E)-010  The Analysis of Phospholipids in Mouse Brain by HPLC/ESI-LIT-TOF MS (PDF, 348KB)  The cell membrane, composed of a variety of lipids, is involved with cellular signal transduction. For example, glycolipids act as receptors and ligands on the cell membrane and as signal transduction molecules, which aggregate to form a platform for signal transduction referred to as the lipid microdomain, or raft.  Model:NanoFrontier NF/AN(E)-009  Impurity Analysis of Erythromycin by NanoFrontier LD (PDF, 375KB)  The NanoFrontier LD is a hybrid LC/MS/MS system consisting of a linear ion trap and a time-of-flight (TOF) mass analyzer. The linear ion trap allows MSn analysis and the TOF analyzer affords high mass-accuracy/resolution over a wide, quantitative dynamic range. This combination provides a highly reliable system for structure analysis with quantitative capabilities.  Model:NanoFrontier NF/AN(E)-008  Biomarker Discovery in Serum Protein by Combining 2D-nanoLC/LIT-TOFMS and Information Based Acquisition (PDF, 415KB)  To provide an effective tool for biomarker research in clinical proteomics, we developed a platform for discovering biomarker by combining a two dimensional liquid chromatograph (2D-nanoLC), a linear-ion-trap time-of-flight mass spectrometer (LIT-TOF), and an Information Based Acquisition (IBA) function.  Model:NanoFrontier NF/AN(E)-007  Structural Assignment of Oligosaccharides using Mass Spectrometry(Part 2): Direct Structural Assignment of N-linked and O-linked glycopeptides by MSn Mass Spectrometry (PDF, 853KB)  Over 50% of proteins in general, and over 90% of proteins on cell surface, exist as glycoproteins, that is, proteins post-translationally modified with glycans. Those modifying glycans are not only directly involved in the structural stability and mechanism of the proteins, but also play antenna-like roles in the cell-to-cell interaction and signal transduction.  Model:NanoFrontier NF/AN(E)-006  Application of LC/MS/MS analysis to toxicological studies (PDF, 378KB)  A common conventional method for protein expression analysis in toxicological studies is to separate the expressed proteins by two-dimensional electrophoresis (2DE) and identify the differences between samples. The throughput of this technique, however, is limited by separation reproducibility and time consuming procedures.  Model:NanoFrontier NF/AN(E)-005  Structural Assignment of Oligosaccharides using Mass Spectrometry (PDF, 776KB)  Mass spectrometry analyses of various N-glycans binding to proteins/peptides are highly desirable for elucidating their biological activities. An approach based on CID MSn spectra (called MSn spectral matching) acquired by ESI-IT MS and ESI-IT TOF MS in both positive and negative ion modes has been proposed as a simple structural assignment method for N-glycans with and without cleaving from the peptide.  Model:NanoFrontier NF/AN(E)-004  Phosphoproteome Analysis by Mass Spectrometry using IMAC Enrichment and Dephosphorylation(PDF, 369KB)  Protein phosphorylation plays a critical role in intracellular signal transduction; as such, it is one of the most frequently studied post translational modifications. For example, when a cell membrane receptor is stimulated, tyrosine kinase is activated, which causes the phosphorylation of proteins downstream in the signal pathway and the subsequent activation of transcription or cytoskeletal remodeling.  Model:NanoFrontier NF/AN(E)-003  Structural Assignments of N-glycans and identification of peptide sequence of N-glycopeptides using nanoESI/Linear-IT-TOF MS and MSn spectral matching. (PDF, 437KB)  Positive- and negative-ion MSn spectra of N-linked glycopeptides binding a neutral and a sialylated glycan were acquired by using electrospray linear-ion trap time-of-flight mass spectrometry (ESI-LITTOFMS) and collision-induced dissociation (CID) with He as a collision gas.  Model:NanoFrontier NF/AN(E)-002  Identification of a Low-Abundant Biomarker in Human Serum Using nanoLC/LIT-TOFMS and Information Based Acquisition Techniques (PDF, 647KB)  Serum protein profiling using mass spectrometry is one of the most promising approaches for biomarker identification. We adopted a nanoLC/LIT-TOFMS system and newly developed software known as Information Based Acquisition (IBA) to identify biomarkers in human serum.  Model:NanoFrontier NF/AN(E)-001  Assignment of sialylated oligosaccharide isomers (N-Glycans) by using MSn spectral matching (PDF, 419KB)  The four isomers of 2-aminopyridine (PA)-derivatized monosialylated oligosaccharides (complex-type Nglycans) were analyzed using nanoHPLC/ESI-ITTOFMS in the negative-ion mode. It was shown that the negative-ion MS2 and MS3 spectra are reproducible; this is an essential element for MSn spectral matching.  Model:NanoFrontier

	NanoFrontier Liquid Chromatograph Mass Spectrometer ~NanoLC/Linear-Trap-TOF~