Hitachi High-Technologies
Skip to header Hitachi High-Technologies
 News & Events       Sitemap

Go HITACHI Top
global navigation start   | Home | About Us | Investor Relations | Global Network |  Products & Services | Customer Service |

 
Life Science > Products > HPLC/Amino Acid Analyzer > Chromaster
 
contents start

Hitachi High Performance Liquid Chromatograph
Chromaster
- Analysis of decomposition products in aspirin by application of two-wavelength detection -

Request Quote

 Chromaster overview
  The Chromaster 5410 (UV Detector) permits two-wavelength simultaneous measurements by realtime wavelength-switching. Beyond the measurement of impurities, the system provides a powerful tool for the analysis of proteins and peptides. By setting a data capture interval of 400 ms or greater for wavelength-switching, users can accurately detect peaks without missing any data points.
The following is an example of analysis that was conducted.
 

Analysis of decomposition products in an aspirin-based drug


  Sample:
Drug containing aspirin (acetyl salicylic acid)
 
  As an important mechanism of action, reportedly aspirin suppresses the biosynthesis of prostaglandin and exhibits analgesic, fever-lowering, and anti-inflammatory actions. As a fever-lowering analgesic drug, aspirin is widely used both in prescription and over-the-counter drugs, and it is also contained in general common cold remedies.  
  Acetyl salicylic acid & Salicylic acid  
  In aqueous solution, aspirin is known to undergo decomposition by hydrolysis into salicylic acid, and it is reported that the decomposition reaction is promoted at high temperatures, in alkaline solutions, and in the presence of magnesium. (Because aspirin, even in a reagent, contains trace quantities of salicylic acid, it must be handled with care when used as a standard compound.)  

  Preparation of sample:
Ultra-deionized water, 1,000 mL, was added to one tablet, and the tablet was disintegrated by ultrasound treatment. After that, the mixture was filtered with a membrane filter and used as a sample.
Chromaster system  
  System configuration:
 5110 Pump
 5210 AutoSampler
 5310 Column Oven
 5420 UV Detector
・Empower2 Data Processing System
 

  Results of analysis:  
  Data collection interval 400 ms  
Example of analysis result of medicine made of aspirin content

  Conditions:  
 
Column HITACHI LaChrom C18(5 µm)(4.6 mmI.D.×150 mm)
Mobile phase 20 mM CH3COONH4 / CH3OH=80 / 20
Flow rate 1.0 mL/min
Column Temperature 40
Detection UV 205 nm、270 nm
Injection vol. 20 µL
 

  The system is or esearchuse only, and is not intended for any animal or human therapeutic or diagnostic use.Whereas minute peaks associated with decomposition products and additives cannot be detected accurately at 270 nm, which is the optimal measurement wavelength for aspirin (USP, Japan Pharmacopeia: Elution test), by simultaneous detection at 205 nm, micro-peaks such as decomposition products and additives can be verified at the same time.  

  PDF Please see here of the print. (PDF, 141KB)  

  NOTE: These data are an example of measurement; the individual values cannot be guaranteed.  

Related Products
Hitachi High Performance Liquid Chromatograph Chromaster  Hitachi High Performance Liquid Chromatograph Chromaster
Hitachi HPLC Columns LaChrom Series  Hitachi HPLC Columns LaChrom Series

Request Quote
contents end
Search by Google
 > Advanced Search
Life science

Topics

Global Network

Products

starting of secondary navigation UV-Visible/NIR Spectrophotometer

starting of secondary navigation Fluorescence Spectrophotometer

starting of secondary navigation Atomic Absorption Spectrophotometer

HPLC/Amino Acid Analyzer

Chromaster

starting of secondary navigation Chromaster Driver for Waters Empower2 Software

starting of secondary navigation Chromaster system Manager

starting of secondary navigation Primaide

starting of secondary navigation L-8900

starting of secondary navigation HPLC Columns LaChromUltra II C18

starting of secondary navigation HPLC Columns LaChrom Series

starting of secondary navigation Clinical Analyzer

starting of secondary navigation Potentiometric Titrator

starting of secondary navigation Karl Fischer Titrator

starting of secondary navigation Mercury Analyzer

Applications

Contact Us

Sitemap

HPLC Basic Course
pagetop
 
starting of footer  | Terms of use | Privacy Policy | All Rights Reserved Copyright (C) 2001, 2014. Hitachi High-Technologies Corporation.
starting of footer  | Terms of use | Privacy Policy | All Rights Reserved Copyright (C) 2001, 2012. Hitachi High-Technologies Corporation.