Hitachi High Performance Liquid Chromatograph Chromaster - Analysis of decomposition products in aspirin by application of two-wavelength detection -

Chromaster overview

The Chromaster 5410 (UV Detector) permits two-wavelength simultaneous measurements by realtime wavelength-switching. Beyond the measurement of impurities, the system provides a powerful tool for the analysis of proteins and peptides. By setting a data capture interval of 400 ms or greater for wavelength-switching, users can accurately detect peaks without missing any data points. The following is an example of analysis that was conducted.

Analysis of decomposition products in an aspirin-based drug

	Sample: Drug containing aspirin (acetyl salicylic acid)
	As an important mechanism of action, reportedly aspirin suppresses the biosynthesis of prostaglandin and exhibits analgesic, fever-lowering, and anti-inflammatory actions. As a fever-lowering analgesic drug, aspirin is widely used both in prescription and over-the-counter drugs, and it is also contained in general common cold remedies.
	In aqueous solution, aspirin is known to undergo decomposition by hydrolysis into salicylic acid, and it is reported that the decomposition reaction is promoted at high temperatures, in alkaline solutions, and in the presence of magnesium. (Because aspirin, even in a reagent, contains trace quantities of salicylic acid, it must be handled with care when used as a standard compound.)

	Preparation of sample: Ultra-deionized water, 1,000 mL, was added to one tablet, and the tablet was disintegrated by ultrasound treatment. After that, the mixture was filtered with a membrane filter and used as a sample.
	System configuration:  5110 Pump  5210 AutoSampler  5310 Column Oven  5420 UV Detector ・Empower2 Data Processing System

	Results of analysis:
	Data collection interval 400 ms

	Conditions:
	Column HITACHI LaChrom C18(5 µm)(4.6 mmI.D.×150 mm) Mobile phase 20 mM CH3COONH4 / CH3OH=80 / 20 Flow rate 1.0 mL/min Column Temperature 40 Detection UV 205 nm、270 nm Injection vol. 20 µL

The system is or esearchuse only, and is not intended for any animal or human therapeutic or diagnostic use.Whereas minute peaks associated with decomposition products and additives cannot be detected accurately at 270 nm, which is the optimal measurement wavelength for aspirin (USP, Japan Pharmacopeia: Elution test), by simultaneous detection at 205 nm, micro-peaks such as decomposition products and additives can be verified at the same time.

Please see here of the print. (PDF, 141KB)

NOTE: These data are an example of measurement; the individual values cannot be guaranteed.