UHPLC(Ultra High Performance LC) LaChromUltra :Hitachi High-Technologies Corporation

LaChromUltra
High-Speed Liquid Chromatograph

LaChromUltra delivers ultra-fast analysis, higher resolution, increased sensitivity and the flexibility to run both higher pressure and conventional HPLC analyses on a single system.

Topic : HPLC Basic Course 8. Principle and Feature of Various Detection Methods(2)
This chapter is "Fluorescence (FL) detector","Differential refractive index (RI) detector","Conductivity detector","Electrochemical detector (ECD),"Evaporative light scattering detector (ELSD)","Corona® Charged Aerosol Detector® (Corona® CAD®)".

Features	Modules
LaChromUltra C18 Series Columns	Example of system configuration
Applications	HPLC Basic Course New!
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Features

LaChromUltra delivers ultra-fast, high-resolution analyses through a balanced optimization of a 2μm particle column and a maximum 60MPa system pressure.

Ultra-fast analysis times: Reduced to about 1/10, as compared with our conventional HPLC.
Optimized for ultra high-speed analysis
·Detector response times (0.01sec) and sampling periods (10msec) designed for high speed analysis.
·Flulrescence detector: Delivers excellent sensitivity using optimized low-volume flow cell.
·PDA detectoer:A wide wavelength range and excellent resolution make the L-2455U ideal for qualitative analysis.

Excellent reproducibility of small volume injection New!

reproducibility	0.5%RSD or less, 1µL injection*
	0.2%RSD or less, 10µL injection*

(*under a specified condition)

Further improvements for lower carry-over New!

carry-over < 0.003%(one solution wash,methylparaben)*

(*under a specified condition)
Easy to use: finger-tight column switching system allows you to easily change between ultra and conventional LC columns.
Flexible: Choose Hitachi's 3 or 5μm particle columns for easy method transfer between ultra and conventional LC applications on one system.

Analysis time is reduced to 1/10
Example of Analysis of DNPH Aldehyde

Example of Analysis of DNPH-Aldehyde

*1: (4.6mmI.D.×150mm)
*2: (2.0mmI.D.×50mm)

Example of system configuration

Model	Specifications
L-2160U Pump(2 Units)	Dual-plunger reciprocating pump (seriesconnection, pulsation suppression) Max. discharge pressure: 60MPa (0.001 to 2.500mL/min) Flow rate range: 0.001 to 5.000mL/min
Gradient System	2-pump flow rate control
L-2200U Auto Sampler	Withstand pressure: 60MPa Sample injection system: Direct injection Sample injection volume: 0.1 to 20μL(standard loop)
L-2300 Column Oven	Temperature control system Block heating + Air circulation Temperature setting range 1 to 65 (in 1 steps)
L-2400U UV Detector	Optical system: Double-beam ratio photometry system Light source: D₂ lamp, Hg lamp (for wavelength check) Response: 0.01sec (ultra-fast)
Organizer	The organizer is capable of supplying power to two pumps, one auto sampler and one detector. It is also usable as a reservoir to accommodate solvent bottles.
Chromatography Data Station	Empower^TM2 EZChrom Elite^TM
PC OS	Windows®XP

*Windows®XP is registered trademarks, and other company names and merchandise names are registered trademarks or brand names of respective companies.

LaChromUltra High-Speed Liquid Chromatograph

Features

Example of system configuration

LaChromUltra
High-Speed Liquid Chromatograph