HPLC Basic Course

We inform of Basic of HPLC from "Principle and System Configuration of HPLC" to "Merits of Various Detection Methods".

1. Principle and System Configuration of HPLC(1)

Principle of chromatography

2. Principle and System Configuration of HPLC(2)

Philosophy of Qualitative analysis and Quantitative analysis by chromatography      Qualitative analysis      Quantitative analysis

3. Principle and System Configuration of HPLC(3)

Configuration of an HPLC system      Pumping unit      Sample-injection unit      Separation unit      Detection unit      Data-processing unit

4. Separation Mode and Column(1)

		Type of separation mode - Separation depends on the triangle relationships among the sample, filler, and eluate!
		Adsorption mode
		Partition mode

5. Separation Mode and Column(2)

		Type of separation mode - Separation depends on the triangle relationships among the sample, filler, and eluate!(2)
		Ion exchange mode
		Size exclusion mode
		HILIC mode

6. Separation Mode and Column(3)

Selection of eluate

7. Merits of Various Detection Methods(1)

		UV/UV-VIS detectors
		Diode array detector (DAD, PDA: Photodiode Array Detector)

8. Merits of Various Detection Methods(2)

		Fluorescence (FL) detector
		Differential refractive index (RI) detector
		Conductivity detector
		Electrochemical detector (ECD)
		Evaporative light scattering detector (ELSD)
		Corona® Charged Aerosol Detector® (Corona® CAD®)

	Hitachi High Performance Liquid Chromatograph Chromaster
	Hitachi Ultra High-Speed Liquid Chromatograph LaChromUltra