NanoFrontier nLC Nanoflow High Performance Liquid Chromatograph

NanoFrontier nLC -Highly reproducible and stable nanoflow HPLC provides your mass spectrometer more sensitivity and accuracy. NanoFrontier nLC offers true split-less nanoflow gradient formation (from 50nL/min) improving stability of the system and reproducibility of the retention time. NanoFrontier nLC consists of a dedicated gradient pump and a high precision nanoflow pump for stable solvent delivery.

Features  Data  Modules  Standard Specifications  Applications  Contact Us

Features

High reproducibility at low flow rates (from 50nL/min) provided by Hitachi's unique split-less solvent delivery system (DEGS) Easy operation and maintenance obtained by Front Access Integlation with various mass spectrometers

The Hitachi DEGS(Dual Exchange Gradient System, patent pending) technology provides accurate, true split-less nanoflow gradient formation (from 50nL/min)

Washing mode: washes flow route and column efficiently at 1μL/min Stand-by mode: prevents contamination by sending solutions at the minimum rate Quarternary gradient: Four solvents can be used for gradient formation at the maximum Sample cooling function: keeps sample stable On-line deaerating function: deaerates all the solvent used in the system System monitoring function: provides results more reliability by monitoring system conditions during operation. Real-time pressure monitoring: provides information for column condition and system stability in real-time

Data

Gradient reproducibility at a flow rate of 50 nL/min(n=5) [Conditions]       Flow rate: 50 nL/min   Solvents: A - Water; B - 80%CH3CN 50mg/mL   Gradient settings: 0 min(B=10%)→ 40 min(B-100%) Detector UV 273 nm(31-nL cell, 1-nm slit) Continuous measurement reproducibility (200nL/min VS 50nL/min) An example for LC/MS measurement at a flow rate of 50 nL/min [Conditions]       ample: BSA   Sample amount: 1μL   Flow rate: 50 nL/min   Mobile phase: A)0.1% formic acid in 2% acetonitrile     B)0.1% formic acid in 98% acetonitrile   Gradient settings: A/B=98/2(0 min.)→60/40(60 min.)→ 2/98(60,1～70 min.) Retention time reproducibility for each peptide(n=6) Peak No. 1 2 3 4 5 Retention Time(min) 49.08 54.48 57.95 61.26 63.93 m/z 511.63 583.9 653.34 526.28 582.32 Retention Time RSD(%) 0.64 0.63 0.56 0.54 0.55 Peak No. 6 7 8 9 10 Retention Time(min) 64.5 66.66 69.55 71.25 72.73 m/z 473.94 507.84 740.36 820.1 746.35 Retention Time RSD(%) 0.47 0.46 0.41 0.35 0.36 *(Reference) When measuring the same sample by using the conventional LC system at 100 μL/min, the retention time was 0.1% RSD.

Modules

Loading Pump (L-2730) : Pump delivering sample to trap column and washing sample Autosampler (L-2750) : Module injecting sample by direct injection method Gradient Pump (L-2720) : Pump delivering gradient solution stably at 100nL/min Nanoflow Pump (L-2700) : Pump generating reproducible gradient at nanoflow rates (from 50nL/min) Organizer (L-2710) : Power supply for each modules and solvent container holder

Standard Specifications

System Size (with Autosampler) : W 720×L 470×H 650 (mm)Weight 85kg System Size (without Autosampler) : W 340×L 470×H 650 (mm)Weight 46kg Max. Pressure : 20MPa Setup flow rate : 10nL/min～250nL/min (Gradient) 10nL/min～1,000nL/min (Conditioning) Retention time reproducibility : RSD 0.5% (200nL/min) Power supply : AC 100～240V ±10% 50/60Hz Incidental facilities : Humidity 30～80% Temperature : 15～30°C Sample injection 1. Autosampler : standard rack 200 vials, 96 well plate rack (optional) please ask Direct injection Sample injection volume : 0.1～50μL Sample cooler : ～4°C 2. Manual Injector (optional)