UV-Vis/NIR Spectrophotometer Basic Course

We inform of Basic of Spectrophotometer from "What can an UV-Vis Spectrophotometer Do?" to "Mechanism of the Spectrophotometer".

1. What can an Ultraviolet and Visible Spectrophotometer Do?

		Determine the concentration (contents) of a solution
		Determine the characteristics of a material
		Determine molecular structure

2. Colorimetric Analysis

		Light and color
		The relationship between light, radio, and X-rays (about light)
		Sunlight vs. the laser beam (white light and monochromatic light)
		Why is an apple red? (about color)
		Invisible light (ultraviolet and infrared)

3. Colorimetric Analysis

Neglecting cleaning increases absorbance (Bouger-Beer's law).

4. Colorimetric Analysis

		Measurement by color strength (quantitative analysis)
		Sample and standard solution
		Magic drug (coloring reagent)
		Calibration curve

5. Colorimetric Analysis

		See a color (spectrum)
		What's a spectrum?
		What can be determined from a spectrum?

6. Structure of a spectrophotometer

Differences between a man and a spectrophotometer (rough structure)

7. Structure of a spectrophotometer

		Optical system
		Is there a dedicated position for a sample? (Single beam and double beam)
		Careful selection of wavelength (single and double monochromes)

8. Structure of a spectrophotometer

		Components of spectrophotometer
		Light source
		Spectroscope
		Sample cell
		Detector

	U-4100 UV-Visible-NIR Spectrophotometer
	U-3900/3900H Spectrophotometer
	U-2900/2910 Double Beam Spectrophotometer
	U-5100 Ratio Beam Spectrophotometer
	Diffraction Grating