アプリケーションデータ：液体クロマトグラフ質量分析計 （LC/MS）

長谷川英樹・橋本雄一郎・和氣泉H. Hasegawa, Y. Hashimoto, I. Waki
株式会社日立製作所中央研究所Central Research Laboratory, Hitachi, Ltd., Kokubunji, Tokyo 185-8601, Japan

2つのリニアイオントラップ（LIT）と直交加速飛行時間型質量分析計（oaTOF）を結合したDual LIT / oaTOFを開発

• 1mTorr以下の1st LITによる高イオン選択性能


• 50-100mTorrの2nd LITによる高イオン収束性能


• oaTOFによる高質量分解能

Yuichiro Hashimoto, Hideki Hasegawa and Izumi Waki
Central Research Laboratory, Hitachi, Ltd., Tokyo 185-8601, Japan

• Hybrid mass spectrometer conprising dual linear traps (LITs)and an orthogonal acceleration TOF mass spectrometer. (oaTOF)
  
• High selectivity MSn analysis in the 1st LIT
  
• Effective damping in the 2nd LIT
  
• High mass resolution and high mass accuracy detection in the oaTOF

T. Yokosuka1, K. Yoshinari1, K. Kobayashi1, A. Ohtake1, A. Hirabayashi2, Y. Hashimoto2, I. Waki2, Y. Terui3and T. Takao41Hitachi Research Laboratory, Hitachi, Ltd. Hitachi, Ibaraki 319-1292, Japan 2Central Research Laboratory, Hitachi, Ltd. Kokubunji, Tokyo 185-8601, Japan3Hitachi High-Technologies Corporation, Hitachinaka, Ibaraki 312-8504, Japan 4Osaka University, Suita, Osaka 565-0871, Japan

A real-time optimization system for MSn(n=2,3), called IBAIBA( Information Based Acquisition ), was developed.
The achievement of real-time optimal analysis flow control using IBA was confirmed.

A. Hirabayashi1, T. Yokosuka2, K. Yoshinari2, A. Ohtake2, K. Kobayashi2, Y. Hashimoto1, H. Hasegawa1, I. Waki1,N. Manri1and M. Ishimaru11Central Research Laboratory, Hitachi, Ltd., Kokubunji, Tokyo 185-8601, Japan 2Hitachi Research Laboratory, Hitachi, Ltd., Hitachi, Ibaraki 319-1292, Japan

• A real-time optimization technique for MSn(n=2, 3), called IBA, was developed.
  
• The technique was implemented in a nLC/Iontrap-TOFMS.
  
• We tested our technique by repeatedly analyzing a yeast cell lysate, a typical crude sample.

（株）日立ハイテクノロジーズ1・北海道大学大学院理学研究科2
伊藤 裕基1,2・永井 伸治1・吉岡 信二1・武川 泰啓2・出口 喜三郎2・中川 裕章2・篠原 康郎2・西村 紳一郎2

生体内の60%以上のタンパク質は糖鎖修飾されていると言われており、糖鎖の構造とその結合位置の決定は、糖タンパク質の機能を理解する上で重要である。
近年、MALDIやESIをイオン源とする質量分析計（Tandem MS, Ion trap MS, FT MS）による糖タンパク質、糖ペプチドシーケンス解析が急速に進歩しつつある。
しかし、糖鎖部分も含めた完全な構造解析を行うにはまだ多くの課題がある1) 。
今回、新たに開発されたNanoHPLC/ ESI-IT-TOF MSを用いて糖ペプチドのMSnスペクトル構造解析方法の検討を行った。

Yasuhiro Takegawa1, Kisaburo Deguchi1, Shinji Nagai2, Shinji Yoshioka3, Shinya Ito2, Hiroaki Nakagawa1, and Shin-Ichiro Nishimura1
1Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 001-0021, Japan.
2Design & Manufacturing Group, Hitachi High Technologies Co., Hitachinaka, 312-8504, Japan.
3 Naka Customer Center, Hitachi Science Systems Co., Hitachinaka, 312-8504, Japan.

Electrospray ionization mass spectrometry (ESI-MS) and matrixassisted laser desorption ionization/mass spectrometry (MALDI-MS), which are now routinely used in protein/peptide analysis, are becoming indispensable in oligosaccharide analysis as well.
However, despite many attempts, it is still almost impossible tocompletely assign the oligosaccharide structure (i.e., composition, sequence, anomeric character, linkage position, and branching pattern) by MSn analysis alone.
Recently, we demonstrated that MSn spectral matching that is simply based on correlation coefficient calculations between positive or negative-ion MSn spectra is useful and applicable to the structural assignment of relatively large nonfucosylated and fucosylated oligosaccharide isomers (complex typeN-glycans) derivatized by 2-aminopyridine (PA) [1, 2].
In this presentation, we apply MSn spectral matching to assignment of mono-sialylated N-glycan isomers and investigate its usefulness.